The LAS1L Polyclonal Antibody (PACO57616) is a valuable tool for researchers studying LAS1L, a protein involved in ribosome biogenesis and cell cycle regulation. This antibody, produced in rabbits, specifically recognizes LAS1L in human samples and is suitable for use in Western blot applications. By targeting LAS1L, researchers can investigate its role in various cellular processes and its potential implications in cancer and other diseases.LAS1L, a component of the large 60S ribosomal subunit, is essential for proper ribosome assembly and protein synthesis. Dysregulation of LAS1L has been linked to defective ribosomal RNA processing and abnormal cell proliferation, highlighting its importance in maintaining cellular homeostasis.
By studying LAS1L expression and function, researchers can gain insights into its involvement in disease development and potentially identify new therapeutic targets for intervention.Overall, the LAS1L Polyclonal Antibody offers researchers a reliable tool for investigating the biological functions of LAS1L and its relevance in disease pathogenesis. With its specificity and sensitivity in detecting LAS1L, this antibody is well-suited for studies in molecular biology, cell biology, and cancer research.
Western Blot. Positive WB detected in: 293 whole cell lysate. All lanes: LAS1L antibody at 3.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 84, 82, 77, 34 kDa. Observed band size: 84 kDa.
Immunofluorescence staining of HepG2 cells with PACO57616 at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Involved in the biogenesis of the 60S ribosomal subunit. Required for maturation of the 28S rRNA. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.
Synonyms:
Ribosomal biogenesis protein LAS1L (Protein LAS1 homolog), LAS1L
UniProt Protein Function:
LAS1L: a protein of unknown function. Protein type: Nucleolus; RNA-bindingChromosomal Location of Human Ortholog: Xq12Cellular Component: cytoplasm; membrane; microtubule organizing center; nucleoplasmBiological Process: endonucleolytic cleavages during rRNA processing; establishment and/or maintenance of chromatin architecture; maturation of 5.8S rRNA; maturation of LSU-rRNA; rRNA processing
