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Laminin beta 1 Rabbit Polyclonal Antibody (CAB9827)

SKU:
CAB9827
Product Type:
Antibody
Applications:
WB
IHC
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 12 Anti-LAMB1 Antibody CAB9827
  • Cell Biology Antibodies 12 Anti-LAMB1 Antibody CAB9827
€139 - €419
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Description

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Laminin beta 1 Rabbit Polyclonal Antibody (CAB9827)

The LAMB1 Polyclonal Antibody (CAB9827) is a vital tool for researchers studying LAMB1, a key component of laminin, an extracellular matrix protein known for its roles in cell adhesion, migration, and differentiation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blotting applications.Laminin subunit beta-1 (LAMB1) is integral to the structure and function of laminin and is involved in various biological processes, including tissue development and maintenance, neural regeneration, and tumor growth and metastasis. The LAMB1 Polyclonal Antibody binds specifically to the LAMB1 protein, enabling accurate detection and analysis of LAMB1 expression in a variety of cell types.

Research into laminin and its subunits, such as LAMB1, is crucial for understanding the roles of extracellular matrix proteins in health and disease. By investigating the function and regulation of LAMB1, researchers can gain insights into potential therapeutic targets for cancer, neurological disorders, and other conditions involving aberrant cell adhesion and migration. The LAMB1 Polyclonal Antibody is a valuable tool for advancing research in these areas and uncovering new avenues for treatment and intervention.

Product Name:Laminin beta 1 Rabbit Polyclonal Antibody
SKU:CAB9827
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1467-1786 of human Laminin beta 1 (NP_002282.2).
Sequence:EAKL RADE AKQS AEDI LLKT NATK EKMD KSNE ELRN LIKQ IRNF LTQD SADL DSIE AVAN EVLK MEMP STPQ QLQN LTED IRER VESL SQVE VILQ HSAA DIAR AEML LEEA KRAS KSAT DVKV TADM VKEA LEEA EKAQ VAAE KAIK QADE DIQG TQNL LTSI ESET AASE ETLF NASQ RISE LERN VEEL KRKA AQNS GEAE YIEK VVYT VKQS AEDV KKTL DGEL DEKY KKVE NLIA KKTE ESAD ARRK AEML QNEA KTLL AQAN SKLQ LLKD LERK YEDN QRYL EDKA QELA RLEG EVRS LLKD ISQK VAVY STCL
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:5000
Synonyms:CLM; LIS5; Laminin beta 1
Positive Sample:Bewo,Rat heart
Conjugate:Unconjugated
Cellular Localization:Secreted, basement membrane, extracellular matrix, extracellular space.
Calculated MW:198kDa
Observed MW:220kDa

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.

Purification Method:Affinity purification
Gene ID:3912
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using Laminin beta 1 Rabbit pAb (CAB9827) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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