The LacZ Monoclonal Antibody (MACO0616) is a specialized research tool designed for the detection and analysis of beta-galactosidase, a commonly used enzyme in molecular and cellular biology experiments. This antibody, produced using hybridoma technology, exhibits high specificity and sensitivity for detecting LacZ in a variety of samples, including cell lysates and tissue sections.Beta-galactosidase, encoded by the LacZ gene, is widely utilized as a reporter gene in gene expression studies and reporter assays. The LacZ Monoclonal Antibody enables accurate and reliable detection of beta-galactosidase expression, facilitating the study of gene regulation, promoter activity, and protein localization in biological systems.
Whether investigating gene expression patterns, monitoring transfection efficiency, or studying cellular processes, the LacZ Monoclonal Antibody is an essential tool for researchers in molecular biology, genetics, and biotechnology. Its versatility and consistency make it a valuable asset for those studying gene expression and protein localization in a wide range of experimental settings.
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Synonyms:
Beta-galactosidase (Beta-gal) (Lactase), lacZ
Immunofluorescence staining of 293T cells transfected with MACO0616 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody
Immunoprecipitating lacZ in 293T transfected whole cell lysate Lane 1: Mouse control IgG instead of MACO0616 in 293T transfected whole cell lysate. Lane 2: MACO0616 (2µl) + 293T transfected whole cell lysate (500µg) Lane 3: 293T transfected whole cell lysate ( 20µg) For western blotting, the blot was detected with MACO0616 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
Western Blot Positive WB detected in Recombinant protein ( 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg) All lanes: lacZ antibody at 1:2000 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 117 kDa Observed band size: 130 kDa
UniProt Protein Function:
lacZ: Repressed during biofilm formation. [More information is available at EcoGene: EG10527]. galacotsidase is a metalloenzyme exhibiting broad substrate specificity. [More information is available at EcoCyc: EG10527] Protein type: EC 3.2.1.23
UniProt Protein Details:
NCBI Summary:
Repressed during biofilm formation. [More information is available at EcoGene: EG10527]. galacotsidase is a metalloenzyme exhibiting broad substrate specificity. [More information is available at EcoCyc: EG10527].
