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Lactulose Fluorometric Assay Kit (MAES0288)

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SKU:
MAES0288
Product Type:
Assay
Instrument:
Fluorescence Microplate Reader
Detection Method:
Fluorometric
Sample Type:
Milk, Infant Feces
Sensitivity:
4.78 μmol/L
€719

Description

system_update_altDatasheet
Product Name: Lactulose Fluorometric Assay Kit
Product Code: MAES0288
Sample Type: Milk, Infant Feces
Assay Type: Fluorometric
Detection Instrument: Fluorescence microplate reader (Ex/Em=530 nm/590 nm)
Detection Method: Fluorescence method
Sensitivity: 4.78 μmol/L
Detection Range: 4.78-500 μmol/L
Intra-Assay Precision: Three milk samples were assayed in replicates of 20 to determine precision within an assay. (CV = Co-efficient of Variation)
Parameters Sample 1 Sample 2 Sample 3
Mean (μmol/L) 100.0 250.0 350.0
%CV 3.8 3.1 2.6
Inter-Assay Precision: Three milk samples were assayed 20 times in duplicate by three operators to determine precision between assays.
Parameters Sample 1 Sample 2 Sample 3
Mean (μmol/L) 100.0 250.0 350.0
%CV 9.7 8.4 7.4
Recovery: Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 98%.
Standard 1 Standard 2 Standard 3
Expected Conc. (μmol/L) 100.0 250.0 350.0
Observed Conc. (μmol/L) 91 245 364
Recovery Rate(%) 91 98 104
Standard Curve: As the fluroescence value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only.
Lactulose Fluorometric Assay Kit (MAES0288)

Lactulose (4-o-β-galactopyranosyl-D-fructo-furanose), also known as galactoside fructose, 4-β-D-galactosid-D-fructose and lactulose, contains one molecule of galactose and one molecule of fructose. Lactulose is a kind of synthetic disaccharide formed by base isomerization of lactose catalyzed by free amino group of casein during milk heat treatment. Lactulose can produce a specific product under the action of enzyme, which reacts with the chromogenic agent to produce a fluorescence product. The excitation wavelength is 530 nm, and the emission wavelength is 590 nm.

Storage: -20°C
Valid Period: 12 months

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