The Lactic Acid + Histone H3 K18 Monoclonal Antibody is a highly specific antibody designed for research involving histone modifications and cellular metabolism. This antibody is raised in mice and is suitable for use in various applications such as Western blot, immunofluorescence, and immunoprecipitation.Histone H3 K18 is a key histone modification site involved in gene regulation and chromatin structure. Lactic acid, on the other hand, is a metabolite produced during glycolysis and plays a role in cellular metabolism and signaling pathways. This monoclonal antibody specifically targets the interaction between lactic acid and histone H3 K18, providing researchers with a valuable tool for studying the impact of metabolites on epigenetic modifications.
Validated for use in both human and mouse samples, this antibody is ideal for studies in cancer biology, metabolism, and epigenetics. By targeting the intersection between cellular metabolism and epigenetic regulation, researchers can gain insights into the molecular mechanisms underlying diseases such as cancer and metabolic disorders. Additionally, understanding the role of lactic acid and histone modifications in gene expression can provide new avenues for therapeutic interventions in these disease contexts.
Product Name:
Lactic acid-Histone H3-K18 Monoclonal Antibody
SKU:
CAB21214
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic lactylated peptide around K18 of human Lactic acid-Histone H3-K18 (NP_003520.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Clone Number:
ARC53046
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Lactic acid-Histone H3-K18 Rabbit mAb (CAB21214) at1:2000 dilution.NIH/3T3 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
