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L-Lactic Acid/Lactate Assay Kit - Colorimetric (MAES0056)

SKU:
MAES0056
Product Type:
Assay
Detection Method:
Colorimetric
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Tissue, Cells
Sensitivity:
0.10 mmol/L
Range:
0.12-7.0 mmol/L
Assay Time:
30 min
Shipping:
Gel Pack
Research Area:
Food Safety & Analysis
Diabetes & Obesity
Glycolysis & Carbohydrates
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€439
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Description

system_update_altDatasheet
Product Name:L-Lactic Acid/Lactate Assay Kit - Colorimetric
Product Code:MAES0056
Product Size:96 Assays
Sample Type:Serum, plasma, cell culture supernatant, tissue, cells
Assay Time:30 min
Instrument:Microplate reader
Sensitivity:0.10 mmol/L
Detection Range:0.12-7.0 mmol/L
Recovery Rate:105
Inter CV:3.5
Inter CV:1.4

Lactic acid is an intermediate product of glucose metabolism in the body, which is mainly produced by red blood cells, striated muscle and brain tissue. The concentration of lactic acid in the blood mainly depends on the synthesis speed and metabolic rate of liver and kidney. The bidirectional conversion of lactic acid and pyruvate is regulated by lactate dehydrogenase (LDH).

Using NAD+ as H+ receptor, LDH catalyzes the reaction of lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of lactic acid can be calculated.

Shelf Life:6 Months
Storage:2-8°C
Shipping:Gel Pack
Research Area:Glycolysis & Carbohydrates
Neto et al.2P-FLIM unveils time-dependent metabolic shifts during osteogenic differentiation with a key role of lactate to fuel osteogenesis via glutaminolysis identifiedbioRxiv 2023View Citation

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