L- Lactate Assay Kit (Colorimetric) (BA0091)

SKU:: BA0091
UPC:
Product Type:: Assay
Detection Method:: Colorimetric
Instrument:: Microplate Reader
Sample Type:: Serum, Plasma, Cell Culture Media
Research Area:: TCA Cycle; Diabetes & Obesity; Food Safety & Analysis; Plant & Environmental Stress

€939

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Description

Description

L- Lactate Assay Kit - Information

Assay Genie's colorimetric L- lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

Applications

For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.

L- Lactate Assay Kit- Key Features

Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.
High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

L- Lactate Assay Kit - Data Sheet
Method of Detection	OD565nm
Detection Limit	0.05 mM
Samples	Serum, plasma, cell culture media etc
Species	All
Protocol Length	20 min
Size	100 tests
Shelf Life	6 months

More Details

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.