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L Antibody (PACO61874)

SKU:
PACO61874
Product Type:
Antibody
Reactivity:
Virus
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
€359

Description

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L Antibody (PACO61874)

The L Antibody (PAC061874) is a high-quality polyclonal antibody designed for research involving the L protein, a key player in various biological processes. This antibody, generated in rabbits, is specifically reactive with human samples and has been validated for use in Western blot applications. By binding to the L protein, this antibody enables precise detection and analysis in a variety of cell types, making it well-suited for studies in virology, molecular biology, and infectious disease research.The L protein is a crucial component of several viruses and is involved in viral replication, transcription, and packaging.

By targeting the L protein, researchers can gain valuable insights into the mechanisms underlying viral infection and pathogenesis. This antibody is an essential tool for investigating the role of the L protein in viral life cycles, host-virus interactions, and antiviral drug development.Overall, the L Antibody (PAC061874) is a valuable resource for scientists studying viral biology and seeking to understand the molecular basis of viral infections. Its high specificity and sensitivity make it a reliable tool for elucidating the functions of the L protein and advancing research in virology and infectious diseases.

Antibody Name:L Antibody (PACO61874)
Antibody SKU:PACO61874
Size:50ul
Host Species:Rabbit
Tested Applications:ELISA, WB
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:500-1:5000
Species Reactivity:Vesicular stomatitis Indiana virus
Immunogen:Recombinant Vesicular stomatitis Indiana virus RNA-directed RNA polymerase L protein (598-784AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern Blot. Positive WB detected in Recombinant protein. All lanes: L antibody at 7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 28 kDa. Observed band size: 28 kDa.
Background:RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both acticities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.
Synonyms:RNA-directed RNA polymerase L (Protein L) (Large structural protein) (Replicase) (Transcriptase) [Includes: RNA-directed RNA polymerase (EC 2.7.7.48); mRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.56); GDP polyribonucleotidyltransferase (EC 2.7.7.88); Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2 (EC 2.1.1.296)], L
UniProt Protein Function:Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals ().
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q8B0H0
NCBI GenInfo Identifier:81945898
NCBI Gene ID:
NCBI Accession:Q8B0H0.1
UniProt Secondary Accession:Q8B0H0
UniProt Related Accession:Q8B0H0
Molecular Weight:25.4 kDa
NCBI Full Name:RNA-directed RNA polymerase L
NCBI Synonym Full Names:
NCBI Official Symbol:
NCBI Official Synonym Symbols:
NCBI Protein Information:
UniProt Protein Name:RNA-directed RNA polymerase L
UniProt Synonym Protein Names:Large structural protein; Replicase; TranscriptaseIncluding the following 3 domains:RNA-directed RNA polymerase (EC:2.7.7.48); mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56); mRNA guanylyltransferase (EC:2.7.7.-)
Protein Family:
UniProt Gene Name:L  
UniProt Entry Name:L_VSIVC
Secondary Antibody
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
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