KY Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IHC, IF, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ug format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Product Name:
KY Antibody
SKU:
PACO54318
Size:
50μg
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant Human Kyphoscoliosis peptidase protein (23-170AA)
Immunogen Species:
Homo sapiens (Human)
Uniprot No:
Q8NBH2
Form:
Liquid
Tested Applications:
ELISAWBIHCIF
Recommended Dilution:
Application
Recommended Dilution
WB
1:500-1:5000
IHC
1:20-1:200
IF
1:50-1:200
Synonyms:
KY antibody, KY_HUMAN antibody, Kyphoscoliosis peptidase antibody
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate All lanes: KY antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 64, 42, 73, 76 kDa Observed band size: 64 kDa
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using PACO54318 at dilution of 1:100
Immunofluorescence staining of Hela cells with PACO54318 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
