The KY Antibody (PACO54318) is a highly specific and sensitive tool designed for researchers studying CD300A, a vital cell surface molecule involved in immune regulation. This polyclonal antibody, produced in rabbits, is especially reactive with human samples, making it ideal for Western blot applications. By binding to the CD300A protein, researchers can effectively detect and analyze its expression in various cell types, making it an essential tool for immunology and cancer research studies.CD300A, also known as an immune inhibitory receptor, plays a crucial role in maintaining immune homeostasis by regulating inflammation and suppressing allergic reactions.
Its role in modulating immune responses highlights its importance in research focusing on diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions. By gaining a deeper understanding of CD300A's activity, researchers can potentially develop new therapies that target or modulate immune function in the context of these diseases.
Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate. All lanes: KY antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 64, 42, 73, 76 kDa. Observed band size: 64 kDa.
Immunofluorescence staining of Hela cells with PACO54318 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using PACO54318 at dilution of 1:100.
Background:
Probable cytoskeleton-associated protease required for normal muscle growth. Involved in function, maturation and stabilization of the neuromuscular junction. May act by cleaving muscle-specific proteins such as FLNC (By similarity).
Synonyms:
Kyphoscoliosis peptidase (EC 3.4), KY
UniProt Protein Function:
KY: Probable cytoskeleton-associated protease required for normal muscle growth. Involved in function, maturation and stabilization of the neuromuscular junction. May act by cleaving muscle-specific proteins such as FLNC. Belongs to the transglutaminase-like superfamily. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Cell development/differentiation; EC 3.4.-.-; Cell adhesion; ProteaseChromosomal Location of Human Ortholog: 3q22.2Cellular Component: cytoskeleton; Z discMolecular Function: peptidase activityBiological Process: muscle development; proteolysis; neuromuscular junction development
