The KTI12 Polyclonal Antibody (PACO61089) is a vital tool for researchers studying KTI12, a protein involved in RNA modification and translation regulation. This antibody, generated in rabbits, is highly specific to human samples and is validated for use in immunofluorescence and immunohistochemistry applications. Its ability to target the KTI12 protein facilitates the detection and analysis of this crucial molecule in various cell types, making it essential for studies in molecular biology and cancer research.
KTI12, also known as tRNA nucleotidyltransferase, plays a crucial role in maintaining genomic stability and protein synthesis fidelity by ensuring proper tRNA modification and maturation. Dysregulation of KTI12 has been implicated in various diseases, including cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target. By elucidating the function of KTI12, researchers can uncover new insights into RNA biology and potentially develop novel treatments for these disorders.
IHC image of PACO61089 diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO61089 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot. Positive WB detected in: Hela whole cell lysate, SH-SY5Y whole cell lysate, 293T whole cell lysate, U87 whole cell lysate, PC-3 whole cell lysate, Rat brain tissue. All lanes: KTI12 antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 kDa. Observed band size: 39 kDa.
