The KRT8 Polyclonal Antibody (CAB1024) is a valuable tool for researchers studying keratin 8, a type II cytoskeletal protein expressed in epithelial tissues. This antibody, generated in rabbits, specifically targets KRT8 and has been validated for use in various applications, including Western blot analysis.KRT8 is involved in maintaining the structural integrity of epithelial cells and has been implicated in cell signaling pathways, cell migration, and stress responses.
Dysregulation of KRT8 expression has been linked to various diseases, including cancer, liver disease, and inflammatory disorders.By using the KRT8 Polyclonal Antibody, researchers can accurately detect and measure KRT8 levels in different cell types and tissues, providing valuable insights into the role of this protein in disease pathogenesis. This antibody is a crucial tool for studies in cell biology, cancer research, and molecular pathology.
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3856
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse large intestine, using Cytokeratin 8 (KRT8) Rabbit pAb (CAB1024) at 1:4000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
