The KRT76 Monoclonal Antibody (PACO60749) is a powerful tool for research involving KRT76, a protein belonging to the keratin family that is commonly expressed in hair follicles and skin cells. This antibody, developed using a hybridoma technique, exhibits high specificity and sensitivity when detecting KRT76 in human samples, making it ideal for immunohistochemistry and immunofluorescence applications.KRT76 plays a crucial role in maintaining the structural integrity of hair and skin, making it a key target for studying hair growth disorders, skin diseases, and wound healing processes.
By using the KRT76 Monoclonal Antibody, researchers can effectively visualize and analyze KRT76 expression patterns in various cell types and tissues, providing valuable insights into the molecular mechanisms underlying these conditions.
Western Blot. Positive WB detected in: PC-3 whole cell lysate. All lanes: KRT76 antibody at 4.9µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 66 kDa. Observed band size: 66 kDa.
Immunofluorescence staining of SH-SY5Y cells with PACO60749 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO60749 diluted at 1:400 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Probably contributes to terminal cornification.
Synonyms:
Keratin, type II cytoskeletal 2 oral (Cytokeratin-2P) (CK-2P) (K2P) (Keratin-76) (K76) (Type-II keratin Kb9), KRT76, KRT2B KRT2P
