The KRT6A Polyclonal Antibody (CAB7560) is a valuable tool for researchers studying keratin 6A, a protein involved in the structural support of epithelial cells. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By specifically binding to KRT6A, this antibody allows for the detection and analysis of the protein in various cell types, making it ideal for research in dermatology, cancer biology, and tissue regeneration.Keratin 6A is a key component of intermediate filaments in epithelial cells, providing strength and resilience to these tissues.
Dysregulation of KRT6A expression has been linked to various skin disorders, such as psoriasis and skin cancer, as well as wound healing processes. Understanding the role of KRT6A in these conditions is essential for developing targeted therapies that can effectively modulate keratin expression and promote tissue repair. By using the KRT6A Polyclonal Antibody, researchers can gain valuable insights into the function of this protein and its implications in physiological and pathological processes.
Product Name:
Cytokeratin 6 (KRT6) Rabbit Polyclonal Antibody
SKU:
CAB7560
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 6 (KRT6) (NP_005545.1).
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Purification Method:
Affinity purification
Gene ID:
3853
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Cytokeratin 6 (KRT6) Rabbit pAb antibody (CAB7560) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
