The KRT5 Polyclonal Antibody (CAB2662) is a valuable tool for researchers studying keratin 5, a type II cytokeratin expressed in basal cells of stratified epithelia. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically targets the KRT5 protein, allowing for accurate detection and analysis in a variety of cell types.KRT5 plays a crucial role in maintaining the structural integrity of epithelial tissues and is commonly used as a marker for identifying basal cells in skin, hair follicles, and other epithelial structures.
Dysregulation of KRT5 expression has been implicated in various skin disorders and cancers, making it a key focus in dermatology and oncology research.By utilizing the KRT5 Polyclonal Antibody, researchers can gain valuable insights into the expression patterns and functions of KRT5 in normal and disease states. This antibody is an essential tool for studies aimed at understanding the roles of KRT5 in skin biology, wound healing, and cancer progression.
Product Name:
Cytokeratin 5 (KRT5) Rabbit Polyclonal Antibody
SKU:
CAB2662
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 5 (KRT5) (NP_000415.2).
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Purification Method:
Affinity purification
Gene ID:
3852
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cytokeratin 5 Rabbit pAb (CAB2662) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
