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Cytokeratin 5 (KRT5) Rabbit Polyclonal Antibody (CAB2662)

SKU:
CAB2662
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 8 Anti-KRT5 Antibody CAB2662
  • Cell Biology Antibodies 8 Anti-KRT5 Antibody CAB2662
  • Cell Biology Antibodies 8 Anti-KRT5 Antibody CAB2662
  • Cell Biology Antibodies 8 Anti-KRT5 Antibody CAB2662
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Cytokeratin 5 (KRT5) Rabbit Polyclonal Antibody
SKU:CAB2662
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 5 (KRT5) (NP_000415.2).
Sequence:MSRQ SSVS FRSG GSRS FSTA SAIT PSVS RTSF TSVS RSGG GGGG GFGR VSLA GACG VGGY GSRS LYNL GGSK RISI STSG GSFR NRFG AGAG GGYG FGGG
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:K5; CK5; DDD; DDD1; EBS1; EBS2; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; KRT5A; Cytokeratin 5 (KRT5)
Positive Sample:A375,PC-3
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol, extracellular exosome, nucleus
Calculated MW:62kDa
Observed MW:62kDa

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Purification Method:Affinity purification
Gene ID:3852
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Cytokeratin 5 Rabbit pAb (CAB2662) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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