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Anti-KRT5/KRT6A Antibody (MACO0502)

SKU:
MACO0502
Product Type:
Antibody
Reactivity:
Human
Host Species:
Mouse
Isotype:
IgG1, Kappa
Applications:
ELISA
IHC
Antibody Type:
Monoclonal Antibody
Conjugation:
Unconjugated
  • Anti-KRT5/KRT6A Antibody MACO0502
  • Anti-KRT5/KRT6A Antibody MACO0502
€359

Description

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Anti-KRT5/KRT6A Antibody (MACO0502)

In a similar vein, the KRT5/KRT6A Monoclonal Antibody (MACO0502) from Assay Genie is ideal for researchers studying keratin 5 (KRT5) and keratin 6A (KRT6A) proteins in various cell types. This monoclonal antibody was developed specifically for use in Western blot applications and is highly reactive with human samples.KRT5 and KRT6A are intermediate filament proteins that play a crucial role in maintaining the structural integrity of epithelial cells. Aberrant expression or mutations in these keratins are associated with various skin disorders, including epidermolysis bullosa simplex and pachyonychia congenita.

By targeting KRT5 and KRT6A with this monoclonal antibody, researchers can effectively detect and analyze these proteins in different cell types, shedding light on their functions and potential implications in disease pathology.Overall, the KRT5/KRT6A Monoclonal Antibody (MACO0502) serves as a valuable tool for investigating the roles of KRT5 and KRT6A in skin biology, cancer research, and other relevant fields. Its specificity and reliability make it a trusted reagent for studies aimed at understanding the intricate mechanisms underlying epithelial cell function and disease development.

Product Name:Anti-KRT5/KRT6A Antibody
Product Sku:MACO0502
Size:50ul
Host Species:Mouse
Tested Applications:ELISA, IHC
Recommended Dilutions:IHC:1:20-1:200
Species Reactivity:Human
Immunogen:Synthesized peptide derived from human Cytokeratin 5/6
Form:Liquid
Storage Buffer:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Clonality:Monoclonal
Isotype:IgG1, Kappa
Conjugate:Non-conjugated
Background:cytoplasm, cytosol, extracellular exosome, intermediate filament, keratin filament, membrane, nucleus, scaffold protein binding, structural constituent of cytoskeleton, cornification
Synonyms:Keratin, type II cytoskeletal 5 (58 kDa cytokeratin) (Cytokeratin-5) (CK-5) (Keratin-5) (K5) (Type-II keratin Kb5), KRT5
Product ImageIHC image of MACO0502 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
Product ImageIHC image of MACO0502 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
UniProt Protein Function:K5: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP. Belongs to the intermediate filament family.
UniProt Protein Details:

Protein type:Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: mitochondrion; membrane; cytoplasm; keratin filament; plasma membrane; intermediate filament; cytosol; nucleus

Molecular Function:protein binding; structural constituent of cytoskeleton

Biological Process: epidermis development; hemidesmosome assembly

Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dowling-degos Disease 1; Epidermolysis Bullosa Simplex With Migratory Circinate Erythema; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Epidermolysis Bullosa Simplex With Mottled Pigmentation; Epidermolysis Bullosa Simplex, Dowling-meara Type

NCBI Summary:The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
UniProt Code:P13647
NCBI GenInfo Identifier:143811411
NCBI Gene ID:3852
NCBI Accession:P13647.3
UniProt Secondary Accession:P13647,Q6PI71, Q6UBJ0, Q8TA91,
UniProt Related Accession:P13647
Molecular Weight:590
NCBI Full Name:Keratin, type II cytoskeletal 5
NCBI Synonym Full Names:keratin 5
NCBI Official Symbol:KRT5  
NCBI Official Synonym Symbols:K5; CK5; DDD; DDD1; EBS2; KRT5A  
NCBI Protein Information:keratin, type II cytoskeletal 5; CK-5; cytokeratin-5; 58 kda cytokeratin; type-II keratin Kb5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types)
UniProt Protein Name:Keratin, type II cytoskeletal 5
UniProt Synonym Protein Names:58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5
Protein Family:Keratin
UniProt Gene Name:KRT5  
UniProt Entry Name:K2C5_HUMAN

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