The KRT2 Polyclonal Antibody (CAB10375) is a valuable tool for researchers studying keratin 2, a key protein involved in maintaining the structure and integrity of epithelial cells. The antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the KRT2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for studies in dermatology, wound healing, and cancer research.
Keratin 2 is a crucial component of intermediate filaments in epithelial cells, providing strength and resilience to these tissues. Its role in skin, hair, and nail development makes it a target of interest in dermatological research, particularly in understanding conditions such as psoriasis, eczema, and skin cancer. By investigating the function and regulation of KRT2, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for treatment.
Product Name:
KRT2 Rabbit Polyclonal Antibody
SKU:
CAB10375
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Cytokeratin 2e (KRT2) (NP_000414.2).
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Purification Method:
Affinity purification
Gene ID:
3849
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Cytokeratin 2e (KRT2) antibody (CAB10375) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
