The KRT12 Polyclonal Antibody (CAB9642) is a valuable tool for researchers studying keratin 12, a key protein found in the eye's cornea. This antibody, generated in rabbits, has a high affinity for human samples and has been validated for use in Western blot applications. By binding specifically to the KRT12 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an ideal choice for studies focusing on corneal biology and related eye diseases.
Keratin 12 is essential for the structural integrity and function of the cornea, making it crucial for maintaining clear vision. Mutations in the KRT12 gene have been linked to various corneal dystrophies and degenerative conditions, making it a target of interest for researchers in ophthalmology and vision science. Understanding the role of KRT12 in corneal health is essential for developing new strategies for treating and preventing vision disorders related to this protein.
Product Name:
Cytokeratin 12 (KRT12) Rabbit Polyclonal Antibody
SKU:
CAB9642
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 295-494 of human Cytokeratin 12 (Cytokeratin 12 (KRT12)) (NP_000214.1).
KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.
Purification Method:
Affinity purification
Gene ID:
3859
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cytokeratin 12 (KRT12) Rabbit pAb (CAB9642) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
