The Cytokeratin 12 (KRT12) Antibody (CAB14746) is a high-quality antibody developed for reliable detection and analysis of target proteins. KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Cytokeratin 12 (KRT12) Antibody
SKU:
CAB14746
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
K12, MECD1, Cytokeratin 12 (KRT12)
Positive Sample:
Mouse eye, Rat eye
Cellular Localization:
Cytoskeleton, Cytosol, Extracellular Exosome.
Calculated MW:
54kDa
Observed MW:
56kDa
KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.
Purification Method
Affinity purification
Gene ID
3859
RRID
AB_2761623
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cytokeratin 12 (KRT12) Rabbit pAb (CAB14746) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
