The KRT12 Polyclonal Antibody (CAB14746) is a crucial tool for research involving KRT12, a protein responsible for maintaining the structural integrity of the corneal epithelium. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically targeting the KRT12 protein, this antibody enables precise detection and analysis in various cell types, making it an essential component for studies in ophthalmology and corneal disease research.KRT12 is a key component of the cytoskeleton in corneal epithelial cells, contributing to the strength and durability of the cornea.
Mutations in the KRT12 gene have been associated with a range of corneal disorders, including Meesmann corneal dystrophy. Research into the function and regulation of KRT12 is essential for understanding the mechanisms underlying these conditions and developing potential treatments. The KRT12 Polyclonal Antibody offers researchers a valuable tool for investigating the role of KRT12 in corneal health and disease, opening up new possibilities for therapeutic interventions.
Product Name:
Cytokeratin 12 (KRT12) Rabbit Polyclonal Antibody
SKU:
CAB14746
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 295-494 of human Cytokeratin 12 (Cytokeratin 12 (KRT12)) (NP_000214.1).
KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.
Purification Method:
Affinity purification
Gene ID:
3859
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cytokeratin 12 (KRT12) Rabbit pAb (CAB14746) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
