The KRAS Polyclonal Antibody (CAB12704) offered by Assay Genie is a powerful tool for research involving KRAS, a key protein involved in cell signaling pathways and cancer development. This antibody, produced in a rabbit host, is highly specific to human KRAS samples and has been validated for use in Western blot applications. By binding to the KRAS protein, this antibody allows for precise detection and analysis of KRAS expression in various cell types, making it essential for studies in cancer biology and precision medicine.
KRAS, a member of the RAS family of oncogenes, is frequently mutated in various cancers, driving abnormal cell growth and tumor progression. Understanding the role of KRAS in cancer development is crucial for the development of targeted therapies that can effectively inhibit its oncogenic activity. The KRAS Polyclonal Antibody is a valuable tool for researchers aiming to investigate the mechanisms of KRAS signaling and potentially identify new therapeutic targets for cancer treatment.
Product Name:
[KO Validated] KRAS Rabbit Polyclonal Antibody
SKU:
CAB12704
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-188 of human KRAS (NP_203524.1).
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.
Purification Method:
Affinity purification
Gene ID:
3845
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and KRAS knockout (KO) 293T cells, using [KO Validated] KRAS Rabbit pAb (CAB12704) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
