The KPNA2 Rabbit Monoclonal Antibody (CAB5012) is a valuable tool for researchers studying KPNA2, a key protein involved in nucleocytoplasmic transport. This antibody, generated in rabbits, is highly specific for KPNA2 in human samples and has been validated for use in Western blot applications. By binding to the KPNA2 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in cell biology and cancer research.KPNA2, also known as importin alpha-1, plays a crucial role in transporting proteins into the nucleus, a process essential for gene expression and cellular function.
Dysregulation of KPNA2 has been linked to various diseases, including cancer, making it a promising target for therapeutic interventions. By furthering our understanding of KPNA2 function, researchers can work towards developing novel treatments that target nucleocytoplasmic transport mechanisms in disease settings.
Product Name:
KPNA2 Rabbit Monoclonal Antibody
SKU:
CAB5012
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KPNA2 (P52292).
The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3838
Clone Number:
ARC1227
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using KPNA2 Rabbit mAb (CAB5012) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
