The KO Validated Vimentin Rabbit Monoclonal Antibody (CAB19607) is a specialized antibody designed for research involving vimentin, a key protein involved in cell structure and function. This antibody, developed and validated for use in knockout (KO) studies, is highly specific and reactive with vimentin in various cell types.Vimentin is a critical intermediate filament protein that plays a crucial role in maintaining cell shape, integrity, and motility. It is also involved in processes like cell division, migration, and signaling. Studying vimentin using this monoclonal antibody can provide valuable insights into its function and regulation in different biological contexts.
With its high specificity and sensitivity, the KO Validated Vimentin Rabbit Monoclonal Antibody (CAB19607) is ideal for applications such as immunofluorescence, immunohistochemistry, and Western blotting. Researchers in fields like cell biology, cancer biology, and developmental biology can benefit from using this antibody to investigate vimentin's role in various cellular processes and disease states.Overall, this antibody is a valuable tool for scientists looking to delve deeper into vimentin biology and its implications for human health and disease. Its rigorous validation and reliable performance make it a trusted reagent for cutting-edge research in the field.
WB,1:2000 - 1:20000 IHC-P,1:100 - 1:500 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CTRCT30; HEL113; Vimentin; VIM; vimentin; in
Positive Sample:
293T,Jurkat,HeLa,C6,Mouse testis,293T
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
54kDa
Observed MW:
54kDa/
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
Purification Method:
Affinity purification
Gene ID:
7431
Clone Number:
ARC0086
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KD Validated] Vimentin Rabbit mAb (CAB19607) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
