Anti-USP4 Antibody (CAB20005)[KO Validated]
- SKU:
- CAB20005
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Description
Anti-USP4 Antibody (CAB20005)[KO Validated]
The USP4 Polyclonal Antibody (CAB20005) is a reliable tool for researchers studying USP4, a deubiquitinase enzyme that plays a key role in the regulation of various cellular processes. This antibody, derived from rabbits, is highly specific for USP4 in human samples and has been validated for use in Western blotting applications. By binding to USP4, this antibody allows for the detection and examination of this important enzyme in a variety of cell types, making it a valuable tool for studies in molecular biology and cancer research.USP4 is known for its involvement in the regulation of protein stability, DNA repair, and inflammation.
Its dysregulation has been linked to various diseases, including cancer, neurodegenerative disorders, and immune-related conditions. By investigating the function of USP4, researchers can gain insights into the molecular mechanisms underlying these diseases and potentially identify new therapeutic targets for intervention. Overall, the USP4 Polyclonal Antibody offers researchers a high-quality reagent for exploring the functions of USP4 in a variety of research applications.
| Antibody Name: | Anti-USP4 Antibody [KO Validated] |
| Antibody SKU: | CAB20005 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human USP4. |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant protein of human USP4. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 7375 |
| Uniprot: | Q13107 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 35kDa/103kDa/108kDa |
| Observed MW: | 108KDa |
![Anti-USP4 Antibody [KO Validated] (CAB20005)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/l/450/A20005_1__43548.jpg) | Western blot analysis of extracts from normal (control) and USP4 knockout (KO) HeLa cells, using USP4 antibody (CAB20005) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
| Synonyms: | USP4, UNP, Unph |
| Background: | The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | USP4: Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER. Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2. Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung. Expressed in the hippocampal neurons. Belongs to the peptidase C19 family. USP4 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.4.19.12; Oncoprotein; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease Chromosomal Location of Human Ortholog: 3p21.31 Cellular Component: cytoplasm; cytosol; lysosome; nucleus; plasma membrane Molecular Function:adenosine receptor binding; identical protein binding; protein binding; ubiquitin-specific protease activity Biological Process: assembly of spliceosomal tri-snRNP; negative regulation of protein ubiquitination; protein deubiquitination; regulation of protein stability |
| NCBI Summary: | The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | Q13107 |
| NCBI GenInfo Identifier: | 116242839 |
| NCBI Gene ID: | 7375 |
| NCBI Accession: | Q13107.3 |
| UniProt Secondary Accession: | Q13107,O43452, O43453, Q08AK8, A8K6Y0, C9IY91, |
| UniProt Related Accession: | Q13107 |
| Molecular Weight: | 109kDa |
| NCBI Full Name: | Ubiquitin carboxyl-terminal hydrolase 4 |
| NCBI Synonym Full Names: | ubiquitin specific peptidase 4 |
| NCBI Official Symbol: | USP4Â Â |
| NCBI Official Synonym Symbols: | UNP; Unph  |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase 4 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase 4 |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme 4; Ubiquitin thioesterase 4; Ubiquitin-specific-processing protease 4; Ubiquitous nuclear protein homolog |
| UniProt Gene Name: | USP4Â Â |
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