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[KO Validated] UBE2O Rabbit Polyclonal Antibody (CAB10036)

SKU:
CAB10036
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
  • KO Validated Antibodies 1 Anti-UBE2O Antibody CAB10036KO Validated
  • KO Validated Antibodies 1 Anti-UBE2O Antibody CAB10036KO Validated
  • KO Validated Antibodies 1 Anti-UBE2O Antibody CAB10036KO Validated
  • KO Validated Antibodies 1 Anti-UBE2O Antibody CAB10036KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] UBE2O Rabbit Polyclonal Antibody
SKU:CAB10036
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3).
Sequence:LVNE PYYN EAGF DSDR GLQE GYEN SRCY NEMA LIRV VQSM TQLV RRPP EVFE QEIR QHFS TGGW RLVN RIES WLET HALL EKAQ ALPN GVPK ASSS PEPP AVAE LSDS GQQE PEDG GPAP GEAS QGSD SEGG AQGL ASAS RDHT DQTS ETAP DASV PPSV KPKK RRKS YRSF LPEK SGYP DIGF PLFP LSKG FIKS IRGV LTQF RAAL LEAG MPEC TEDK
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:WB,1:1000 - 1:4000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:E2-230K; 2O
Positive Sample:A-431,HepG2,Jurkat,Mouse heart,Mouse brain
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:141kDa
Observed MW:200kDa

Enables ubiquitin conjugating enzyme activity and ubiquitin protein ligase activity. Involved in positive regulation of BMP signaling pathway; protein ubiquitination; and retrograde transport, endosome to Golgi. Located in cytoplasm and nuclear body.

Purification Method:Affinity purification
Gene ID:63893
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] UBE2O Rabbit pAb (CAB10036) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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