The KO Validated UBE2O Polyclonal Antibody (CAB10036) is a specialized tool for research involving UBE2O, a key enzyme in the ubiquitination pathway that is essential for protein degradation and regulation. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it reliable for use in Western blot applications. By targeting the UBE2O protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an invaluable asset for studies in molecular biology and cancer research.UBE2O, also known as ubiquitin-conjugating enzyme E2O, plays a crucial role in maintaining protein homeostasis by tagging proteins for degradation or regulating their activity through ubiquitination.
Its involvement in cellular processes makes it a critical target for investigation in diseases such as cancer, neurodegenerative disorders, and metabolic diseases. By understanding the function of UBE2O, researchers can develop strategies to manipulate protein stability and activity, potentially leading to novel therapeutic interventions in various disease settings.
Product Name:
[KO Validated] UBE2O Rabbit Polyclonal Antibody
SKU:
CAB10036
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3).
WB,1:1000 - 1:4000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
E2-230K; 2O
Positive Sample:
A-431,HepG2,Jurkat,Mouse heart,Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
141kDa
Observed MW:
200kDa
Enables ubiquitin conjugating enzyme activity and ubiquitin protein ligase activity. Involved in positive regulation of BMP signaling pathway; protein ubiquitination; and retrograde transport, endosome to Golgi. Located in cytoplasm and nuclear body.
Purification Method:
Affinity purification
Gene ID:
63893
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] UBE2O Rabbit pAb (CAB10036) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
