The KO Validated UBE2C Polyclonal Antibody (CAB18075) is a valuable tool for researchers studying UBE2C, a crucial enzyme involved in the ubiquitin-proteasome pathway. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for Western blot applications.UBE2C is known for its role in regulating cell cycle progression and maintaining genomic stability. Dysregulation of UBE2C has been implicated in various diseases, including cancer. By targeting UBE2C with this validated antibody, researchers can analyze its expression levels and activity in different cell types, providing insights into its function in cellular processes.
The UBE2C Polyclonal Antibody is a reliable resource for advancing research into the mechanisms underlying cell cycle control and the development of potential therapeutic strategies targeting UBE2C in diseases such as cancer. Its specificity and sensitivity make it a valuable asset for scientists studying ubiquitination pathways and protein degradation mechanisms.
Product Name:
[KO Validated] UBE2C Rabbit Polyclonal Antibody
SKU:
CAB18075
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human UBE2C (NP_008950.1).
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19.
Purification Method:
Affinity purification
Gene ID:
11065
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and UBE2C knockout (KO) HeLa cells, using [KO Validated] UBE2C Rabbit pAb (CAB18075) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
