The KO-Validated TRIM25 Polyclonal Antibody (CAB19887) is a high-quality research tool designed for investigating TRIM25, a key player in the regulation of immune responses and antiviral defense mechanisms. This antibody, produced in rabbits, exhibits strong reactivity with samples from knockout cell lines and is validated for use in various applications, including Western blotting.TRIM25, a member of the tripartite motif (TRIM) family of proteins, functions as an E3 ubiquitin ligase and is involved in the innate immune response to viral infections. Its role in regulating the RIG-I signaling pathway highlights its importance in antiviral immunity and inflammatory responses.
By targeting TRIM25 with this specific antibody, researchers can gain valuable insights into its function and potential as a therapeutic target in conditions such as viral infections and autoimmune diseases.The validation and specificity of the KO-Validated TRIM25 Polyclonal Antibody make it an essential tool for studies in immunology, virology, and molecular biology. Its ability to detect TRIM25 in various cell types and experimental settings makes it a versatile reagent for researchers striving to unravel the intricate mechanisms of immune regulation and antiviral defense.
Product Name:
[KO Validated] TRIM25 Rabbit Polyclonal Antibody
SKU:
CAB19887
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM25 (NP_005073.2).
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein is an RNA binding protein, functions as a ubiquitin E3 ligase and is involved in multiple cellular processes, including regulation of antiviral innate immunity.
Purification Method:
Affinity purification
Gene ID:
7706
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and TRIM25 knockout (KO) A-549 cells, using [KO Validated] TRIM25 Rabbit pAb (CAB19887) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10S.
