Anti-TRIM21 Antibody (CAB18027)

Antibody Name:	Anti-TRIM21 Antibody [KO Validated]
Antibody SKU:	CAB18027
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-330 of human TRIM21 (NP_003132.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-330 of human TRIM21 (NP_003132.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SQEA REGT QGER CAVH GERL HLFC EKDG KALC WVCA QSRK HRDH AMVP LEEA AQEY QEKL QVAL GELR RKQE LAEK LEVE IAIK RADW KKTV ETQK SRIH AEFV QQKN FLVE EEQR QLQE LEKD EREQ LRIL GEKE AKLA QQSQ ALQE LISE LDRR CHSS ALEL LQEV IIVL ERSE SWNL KDLD ITSP ELRS VCHV PGLK KMLR TCAV HITL DPDT ANPW LILS EDRR QVRL GDTQ QSIP GNEE RFDS YPM
Gene ID:	6737
Uniprot:	P19474
Cellular Location:	Cytoplasm, Cytoplasmic vesicle, Nucleus, P-body, autophagosome
Calculated MW:	45kDa/54kDa
Observed MW:	54kDa

Anti-TRIM21 Antibody [KO Validated] (CAB18027)

Western blot analysis of extracts from normal (control) and TRIM21 knockout (KO) A-549 cells, using TRIM21 antibody (CAB18027) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Synonyms:

TRIM21, RNF81, RO52, Ro/SSA, SSA, SSA1

Background:

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

UniProt Protein Function:	TRIM21: E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)- like complex is shown to mediate ubiquitination of CDKN1B ('Thr- 187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin- mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system; EC 6.3.2.-; Ligase; Ubiquitin ligase Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: nucleoplasm; cytoplasm; SCF ubiquitin ligase complex; cytosol; nucleus; ribonucleoprotein complex Molecular Function:identical protein binding; protein binding; DNA binding; zinc ion binding; RNA binding; ubiquitin-protein ligase activity; ligase activity Biological Process: protein monoubiquitination; regulation of interferon type I production; negative regulation of viral transcription; protein autoubiquitination; protein polyubiquitination; positive regulation of virion penetration into host cell; protein destabilization; inhibition of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; positive regulation of interferon type I production; protein ubiquitination; innate immune response; positive regulation of cell cycle; positive regulation of transcription factor activity; cell cycle
NCBI Summary:	This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	P19474
NCBI GenInfo Identifier:	133250
NCBI Gene ID:	6737
NCBI Accession:	P19474.1
UniProt Secondary Accession:	P19474,Q5XPV5, Q96RF8,
UniProt Related Accession:	P19474
Molecular Weight:	475
NCBI Full Name:	E3 ubiquitin-protein ligase TRIM21
NCBI Synonym Full Names:	tripartite motif containing 21
NCBI Official Symbol:	TRIM21
NCBI Official Synonym Symbols:	SSA; RO52; SSA1; RNF81; Ro/SSA
NCBI Protein Information:	E3 ubiquitin-protein ligase TRIM21; SS-A; ro(SS-A); 52 kDa Ro protein; RING finger protein 81; Sicca syndrome antigen A; tripartite motif-containing 21; sjoegren syndrome type A antigen; tripartite motif-containing protein 21; 52 kDa ribonucleoprotein aut
UniProt Protein Name:	E3 ubiquitin-protein ligase TRIM21
UniProt Synonym Protein Names:	52 kDa Ro protein; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; RING finger protein 81; Ro(SS-A); Sjoegren syndrome type A antigen; SS-A; Tripartite motif-containing protein 21
Protein Family:	Serotype-specific antigen
UniProt Gene Name:	TRIM21
UniProt Entry Name:	RO52_HUMAN

Antibodies	ELISA Kits
Anti-TRIM21 Antibody (CAB13547)	Human E3 ubiquitin-protein ligase TRIM21 (TRIM21) ELISA Kit
Anti-TRIM21 Antibody (CAB1957)
TRIM21 Antibody (PACO12907)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)