The TBP Polyclonal Antibody (CAB2192) is a valuable tool for research involving TATA-Box Binding Protein (TBP), a crucial transcription factor that plays a key role in gene expression regulation. The antibody is raised in rabbits and is highly reactive with human samples, making it a reliable tool for Western blot applications.TBP is essential for the initiation of transcription by RNA polymerase II, making it a critical factor in the regulation of gene expression. Dysregulation of TBP has been linked to various diseases, including cancer and neurodegenerative disorders.
Therefore, studying the expression and activity of TBP is essential for understanding these diseases and developing potential therapeutic strategies.With its specificity and sensitivity, the TBP Polyclonal Antibody (CAB2192) enables researchers to detect and analyze TBP expression in various cell types, making it an ideal tool for studies in molecular biology, genetics, and disease research. By targeting TBP, researchers can gain valuable insights into the mechanisms underlying gene regulation and potentially identify new targets for therapeutic intervention.
Product Name:
[KO Validated] TBP Rabbit Polyclonal Antibody
SKU:
CAB2192
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TBP (NP_001165556.1).
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6908
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using TBP Rabbit pAb (CAB2192) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
