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[KO Validated] TBP Rabbit Polyclonal Antibody (CAB2192)

SKU:
CAB2192
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • KO Validated Antibodies 1 Anti-TBP Antibody CAB2192KO Validated
  • KO Validated Antibodies 1 Anti-TBP Antibody CAB2192KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] TBP Rabbit Polyclonal Antibody
SKU:CAB2192
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TBP (NP_001165556.1).
Sequence:MTPG IPIF SPMM PYGT GLTP QPIQ NTNS LSIL EEQQ RQQQ QQQQ QQQQ QQQQ QQQQ QQQQ QQQQ QQQQ QQQQ QQQA VAAA AVQQ STSQ QATQ GTSG QAPQ
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:HDL4; TBP1; GTF2D; SCA17; TFIID; GTF2D1; TBP
Positive Sample:RAW264.7,C6,SK-OV-3
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:38kDa
Observed MW:33-38kDa/35-45kDa

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:6908
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using TBP Rabbit pAb (CAB2192) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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