Anti-TAF12 Antibody (CAB18073)[KO Validated]
- SKU:
- CAB18073
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Anti-TAF12 Antibody (CAB18073)[KO Validated]
The TAF12 Polyclonal Antibody (CAB18073) is a valuable tool for researchers studying TAF12, a key component of the transcription factor IID (TFIID) complex involved in RNA polymerase II transcription. This rabbit polyclonal antibody is highly specific to TAF12 and has been validated for use in various applications, including Western blotting.TAF12 is essential for the assembly and function of the TFIID complex, which is crucial for the initiation of transcription in eukaryotic cells. By targeting TAF12, researchers can gain insights into the regulation of gene expression and identify potential therapeutic targets for diseases related to transcription dysregulation.
The TAF12 Polyclonal Antibody is particularly useful for studies focusing on gene transcription, cellular differentiation, and development. Its high specificity and sensitivity make it a reliable tool for detecting TAF12 expression levels in different cell types and tissues. Researchers working in the fields of molecular biology, genetics, and developmental biology will benefit from using this antibody in their experiments.
| Antibody Name: | Anti-TAF12 Antibody [KO Validated] |
| Antibody SKU: | CAB18073 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human TAF12 (NP_001128690.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human TAF12 (NP_001128690.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNQF GPSA LINL SNFS SIKP EPAS TPPQ GSMA NSTA VVKI PGTP GAGG RLSP ENNQ VLTK KKLQ DLVR EVDP NEQL DEDV EEML LQIA DDFI ESVV TAAC QLAR HRKS STLE VKDV QLHL ERQW NMWI PGFG SEEI RPYK KACT TEAH KQRM ALIR KTTK K |
| Gene ID: | 6883 |
| Uniprot: | Q16514 |
| Cellular Location: | Nucleus |
| Calculated MW: | 14kDa/17kDa |
| Observed MW: | 18kDa |
![Anti-TAF12 Antibody [KO Validated] (CAB18073)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/l/360/A18073_1__24206.jpg) | Western blot analysis of extracts from normal (control) and TAF12 knockout (KO) HeLa cells, using TAF12 antibody (CAB18073) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
| Synonyms: | TAF12, TAF2J, TAFII20 |
| Background: | Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | TAF12: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. Belongs to the TAF12 family. 2 isoforms of the human protein are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:Transcription initiation complex Chromosomal Location of Human Ortholog: 1p35.3 Cellular Component: nucleoplasm; transcription factor TFIID complex; PCAF complex Molecular Function:protein binding; histone acetyltransferase activity; DNA binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity; transcription factor binding Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; viral reproduction; transcription initiation; RNA elongation from RNA polymerase II promoter; positive regulation of transcription factor activity; gene expression |
| NCBI Summary: | Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2008] |
| UniProt Code: | Q16514 |
| NCBI GenInfo Identifier: | 3024708 |
| NCBI Gene ID: | 6883 |
| NCBI Accession: | Q16514.1 |
| UniProt Secondary Accession: | Q16514,Q15775, Q5T077, D3DPM5, |
| UniProt Related Accession: | Q16514 |
| Molecular Weight: | 161 |
| NCBI Full Name: | Transcription initiation factor TFIID subunit 12 |
| NCBI Synonym Full Names: | TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa |
| NCBI Official Symbol: | TAF12Â Â |
| NCBI Official Synonym Symbols: | TAF2J; TAFII20Â Â |
| NCBI Protein Information: | transcription initiation factor TFIID subunit 12; TAFII20/TAFII15; TAFII-20/TAFII-15; transcription initiation factor TFIID 20/15 kDa subunits; TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD |
| UniProt Protein Name: | Transcription initiation factor TFIID subunit 12 |
| UniProt Synonym Protein Names: | Transcription initiation factor TFIID 20/15 kDa subunits; TAFII-20/TAFII-15; TAFII20/TAFII15 |
| Protein Family: | Transcription initiation factor |
| UniProt Gene Name: | TAF12Â Â |
| UniProt Entry Name: | TAF12_HUMAN |
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