The STK4 Polyclonal Antibody (CAB18100) is specifically designed for research involving STK4, a serine/threonine kinase that plays a key role in regulating cell proliferation and survival. This antibody, generated in rabbits, is highly reactive with samples from various species and has been validated for use in Western blot applications. By binding to the STK4 protein, this antibody enables accurate detection and analysis in a wide range of cell types, making it an essential tool for research in molecular biology and cancer biology.
STK4, also known as MST1 (mammalian sterile 20-like kinase 1), is a crucial component of the Hippo signaling pathway, which controls organ size and cell growth. Dysregulation of STK4 has been linked to various cancers and other diseases, highlighting its significance in cellular homeostasis and tumor suppression. Investigating the function of STK4 with this reliable antibody is essential for understanding its role in disease pathogenesis and developing targeted therapies for cancer and other related conditions.
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.
Purification Method:
Affinity purification
Gene ID:
6789
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and MST1/STK4 knockout (KO) HeLa cells, using [KO Validated] MST1/STK4 Rabbit pAb (CAB18100) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
