The KO Validated STAT5B Rabbit Monoclonal Antibody (CAB19567) is a high-quality antibody designed for research on STAT5B, a key transcription factor involved in regulating cell growth and differentiation. This antibody, produced in rabbits, has been rigorously validated for use in various applications, including Western blotting and immunohistochemistry.STAT5B is a critical signaling molecule that plays a crucial role in cellular processes such as proliferation, survival, and differentiation. Dysregulation of STAT5B has been implicated in various diseases, including cancer and immune disorders.
This antibody specifically targets STAT5B, allowing for accurate detection and analysis in a variety of cell types, making it an essential tool for studies in oncology, immunology, and cell biology.By utilizing the KO Validated STAT5B Rabbit Monoclonal Antibody, researchers can gain valuable insights into the function and regulation of STAT5B, paving the way for the development of targeted therapies for diseases associated with STAT5B dysregulation. Its high specificity and sensitivity make it an indispensable tool for investigating the role of STAT5B in health and disease.
Product Name:
[KO Validated] STAT5B Rabbit Monoclonal Antibody
SKU:
CAB19567
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 600-787 of human STAT5B (P51692).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
STAT5; GHISID2; 5B
Positive Sample:
K-562,Mouse thymus,Mouse heart,Rat heart,HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
90kDa
Observed MW:
90kDa
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.
Purification Method:
Affinity purification
Gene ID:
6777
Clone Number:
ARC0046
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and STAT5B knockout (KO) HeLa cells, using [KO Validated] STAT5B Rabbit mAb (CAB19567) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
