The KO Validated STAT3 Rabbit Monoclonal Antibody (CAB19566) is a powerful tool for researchers studying STAT3, a key transcription factor involved in regulating various cellular processes such as cell growth, differentiation, and survival. This antibody, developed using rabbit monoclonal technology, is specifically validated for knockout (KO) studies in STAT3-deficient cells.With its high specificity and sensitivity, this antibody is suitable for various applications including Western blot analysis, immunoprecipitation, and immunofluorescence.
It recognizes and binds to the STAT3 protein, allowing for precise detection and quantification in different experimental settings.By targeting STAT3, researchers can gain insights into its role in signaling pathways related to cancer, inflammation, and immune response. The KO Validated STAT3 Rabbit Monoclonal Antibody is an essential tool for studies aiming to unravel the complex mechanisms underlying these processes and potentially identify new therapeutic targets for related diseases.
Product Name:
[KO Validated] STAT3 Rabbit Monoclonal Antibody
SKU:
CAB19566
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 600-700 of human STAT3 (P40763).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-600μg extracts of whole cells
Synonyms:
APRF; HIES; ADMIO; ADMIO1; T3
Positive Sample:
HeLa,A549,NIH/3T3,C6,Mouse liver
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
88kDa
Observed MW:
86kDa
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.
Purification Method:
Affinity purification
Gene ID:
6774
Clone Number:
ARC2603
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KO Validated] STAT3 Rabbit mAb (CAB19566) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
