The KO Validated SOX2 Rabbit Monoclonal Antibody (CAB19118) is a highly specific tool for studying the SOX2 protein, a key transcription factor involved in stem cell pluripotency and cancer development. This antibody, produced in rabbits, has been rigorously validated for knockout (KO) studies and is suitable for use in techniques such as immunofluorescence and immunohistochemistry.SOX2, a member of the SRY-related HMG-box (SOX) family of transcription factors, plays a crucial role in maintaining the self-renewal and differentiation of stem cells. It is also implicated in various cancers, where its expression levels can impact tumor growth and progression.
The KO Validated SOX2 Rabbit Monoclonal Antibody specifically targets the SOX2 protein, allowing for precise detection and analysis in different experimental settings.Research involving SOX2 is essential for understanding the mechanisms underlying stem cell biology and oncogenesis. By utilizing the KO Validated SOX2 Rabbit Monoclonal Antibody, scientists can explore the functions of SOX2 in normal and diseased tissues, advancing our knowledge of developmental processes and disease mechanisms. This antibody is a valuable tool for investigations in stem cell research, cancer biology, and regenerative medicine.
Product Name:
[KO Validated] SOX2 Rabbit Monoclonal Antibody
SKU:
CAB19118
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SOX2 (NP_003097.1).
Sequence:
MYNM METE LKPP GPQQ TSGG GGGN STAA AAGG NQKN SPDR VKRP MNAF MVWS RGQR RKMA QENP KMHN SEIS KRLG AEWK LLSE TEKR PFID EAKR LRAL
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:
ANOP3; MCOPS3; SOX2
Positive Sample:
Mouse brain,Rat brain,293T
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
34kDa
Observed MW:
38kDa
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
Purification Method:
Affinity purification
Gene ID:
6657
Clone Number:
ARC0449
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SOX2 antibody (CAB19118) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
