The SNAPIN Polyclonal Antibody (CAB19980) is a specialized antibody designed for research involving SNAPIN, a protein involved in regulating vesicle trafficking and fusion in cells. This antibody, developed through rabbit immunization, exhibits high reactivity with human samples and is validated for use in Western blot applications.SNAPIN is a key player in intracellular transport processes, particularly in the fusion of vesicles with target membranes. Its functions in membrane dynamics and protein sorting make it an essential component for cellular trafficking pathways. The SNAPIN Polyclonal Antibody allows for the detection and analysis of SNAPIN in various cell types, making it an ideal tool for researchers studying cellular biology, neuroscience, and endocrine functions.
By targeting SNAPIN, researchers can gain valuable insights into the mechanisms underlying intracellular transport and vesicle fusion events. This antibody's utility in identifying SNAPIN protein levels and localization provides a crucial tool for understanding cellular processes and potential drug targets in diseases related to vesicle trafficking dysregulation. Explore the functions of SNAPIN and its role in cellular physiology with the SNAPIN Polyclonal Antibody (CAB19980).
Product Name:
[KO Validated] SNAPIN Rabbit Polyclonal Antibody
SKU:
CAB19980
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human SNAPIN (NP_036569.1).
The protein encoded by this gene is a coiled-coil-forming protein that associates with the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex of proteins and the BLOC-1 (biogenesis of lysosome-related organelles) complex. Biochemical studies have identified additional binding partners. As part of the SNARE complex, it is required for vesicle docking and fusion and regulates neurotransmitter release. The BLOC-1 complex is required for the biogenesis of specialized organelles such as melanosomes and platelet dense granules. Mutations in gene products that form the BLOC-1 complex have been identified in mouse strains that are models of Hermansky-Pudlak syndrome. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
23557
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and SNAPIN knockout (KO) HeLa cells, using [KO Validated] SNAPIN Rabbit pAb (CAB19980) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
