The SMARCC1 Polyclonal Antibody (CAB6128) is a valuable tool for researchers studying the SMARCC1 protein, a component of the SWI/SNF chromatin remodeling complex that regulates gene expression and DNA repair. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blotting applications.SMARCC1 plays a crucial role in controlling chromatin structure and gene transcription, influencing key cellular processes such as differentiation, proliferation, and DNA damage response. Dysregulation of SMARCC1 expression has been linked to various diseases, including cancer and developmental disorders.
By targeting SMARCC1 with this antibody, researchers can investigate its function in different cell types and gain insights into its role in epigenetic regulation and disease pathogenesis.With its high specificity and sensitivity, the SMARCC1 Polyclonal Antibody is a valuable tool for furthering our understanding of chromatin remodeling and gene regulation mechanisms. Its application in research areas such as cancer biology, epigenetics, and developmental biology makes it an essential asset for scientists studying the molecular mechanisms underlying various diseases and biological processes.
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
HYC5; Rsc8; SRG3; SWI3; BAF155; CRACC1; 55
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
123kDa
Observed MW:
155kDa
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
Purification Method:
Affinity purification
Gene ID:
6599
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and SMARCC1/BAF155 knockout (KO) 293T cells, using [KO Validated] SMARCC1/BAF155 Rabbit pAb (CAB6128) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
