The [KO Validated] SMARCC1/BAF155 Antibody (CAB6128) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KO Validated] SMARCC1/BAF155 Antibody
SKU:
CAB6128
Size:
100μL, 20μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HYC5, Rsc8, SRG3, SWI3, BAF155, CRACC1, 55
Positive Sample:
293T
Cellular Localization:
Nucleus.
Calculated MW:
123kDa
Observed MW:
155kDa
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
Purification Method
Affinity purification
Gene ID
6599
RRID
AB_2863524
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and SMARCC1/BAF155 knockout (KO) 293T cells, using [KO Validated] SMARCC1/BAF155 Rabbit pAb (CAB6128) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg SMARCC1/BAF155 antibody (CAB6128). Western blot was performed from the immunoprecipitate using SMARCC1/BAF155 antibody (CAB6128) at a dilution of 1:1000.
