The SMARCA4 Polyclonal Antibody (CAB0887) is a valuable tool for researchers investigating the function of SMARCA4, a key component of the SWI/SNF chromatin remodeling complex. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot experiments.SMARCA4, also known as BRG1, plays a crucial role in regulating gene expression by remodeling chromatin structure. Dysregulation of SMARCA4 has been linked to various diseases, including cancer, developmental disorders, and immune dysfunction. By targeting SMARCA4 with this antibody, researchers can study its function in different cell types and gain insights into its role in cellular processes such as proliferation, differentiation, and DNA repair.
With its high specificity and reliability in Western blotting, the SMARCA4 Polyclonal Antibody (CAB0887) is an essential tool for researchers in the fields of molecular biology, cancer biology, and epigenetics. Its ability to detect SMARCA4 protein levels in various biological samples makes it a valuable asset for understanding the mechanisms underlying disease pathology and potentially identifying new therapeutic targets.
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6597
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using BRG1/BRG1/SMARCA4 antibody (CAB0887) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
