The SMAD5 Rabbit Monoclonal Antibody (CAB19117) is a cutting-edge tool for researchers studying SMAD5, a key player in the TGF-β signaling pathway that regulates cell growth and differentiation. This antibody, produced using rabbit-derived monoclonal technology, exhibits high specificity and sensitivity towards human samples, making it an invaluable reagent for Western blot applications. By targeting the SMAD5 protein, this antibody enables precise detection and analysis in a wide range of cell types, making it an ideal choice for studies in developmental biology and cancer research.SMAD5 is a vital mediator of the TGF-β signaling cascade, exerting control over a multitude of cellular processes including proliferation, migration, and apoptosis.
Dysregulation of SMAD5 signaling has been implicated in various diseases, such as cancer, cardiovascular disorders, and developmental abnormalities. By delving into the functions of SMAD5, researchers can uncover novel insights into disease mechanisms and potentially identify new therapeutic targets for intervention. The SMAD5 Rabbit Monoclonal Antibody offers a reliable and specific tool for advancing research in these crucial areas.
Product Name:
[KO Validated] Smad5 Rabbit Monoclonal Antibody
SKU:
CAB19117
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Smad5 (Q99717).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
DWFC; JV5-1; MADH5; Smad5
Positive Sample:
K-562,C2C12
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
52kDa
Observed MW:
60kDa
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4090
Clone Number:
ARC0448
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from K-562 cells, using Smad5 Rabbit mAb (CAB19117) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
