The SMAD4 Polyclonal Antibody (CAB18078) is a crucial tool for studying SMAD4, a key signaling protein involved in the TGF-beta pathway that plays a critical role in cell growth and differentiation. This antibody, produced in rabbits, is specifically designed for use in research examining SMAD4 expression and activity in various cell types.SMAD4, also known as Mothers Against Decapentaplegic Homolog 4, is a tumor suppressor gene that is frequently mutated in different cancer types, highlighting its importance in controlling cell proliferation and preventing tumorigenesis. The SMAD4 Polyclonal Antibody enables the detection and analysis of SMAD4 protein levels in Western blot applications, making it an invaluable tool in cancer research and molecular biology studies.
Understanding the function and regulation of SMAD4 is essential for unraveling the complexities of tumor development and progression, as well as potential therapeutic targeting strategies. With its high specificity and sensitivity, the SMAD4 Polyclonal Antibody offers researchers a reliable means of investigating the role of SMAD4 in various biological processes, paving the way for advancements in cancer therapy and personalized medicine.
Product Name:
[KO Validated] Smad4 Rabbit Polyclonal Antibody
SKU:
CAB18078
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 160-450 of human Smad4 (NP_005350.1).
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasing blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
Purification Method:
Affinity purification
Gene ID:
4089
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Smad4 knockout (KO) HeLa cells, using [KO Validated] Smad4 Rabbit pAb (CAB18078) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
