The SMAD4 Rabbit Monoclonal Antibody (CAB19116) is a vital tool for researchers studying the SMAD4 protein, a critical regulator of TGF-beta signaling pathway and crucial in cell growth and differentiation. This antibody, developed in rabbits, exhibits high specificity and sensitivity in detecting SMAD4 in various cell types, making it ideal for research in developmental biology, cancer biology, and stem cell research.SMAD4, also known as Co-SMAD, plays a pivotal role in transducing TGF-beta signals into the nucleus, where it regulates the expression of genes involved in cell proliferation, differentiation, and apoptosis. Dysregulation of SMAD4 has been associated with various diseases, including cancer and developmental disorders.
Understanding the function and regulation of SMAD4 is essential for developing targeted therapies and diagnostic tools in these areas.The SMAD4 Rabbit Monoclonal Antibody provides researchers with a valuable tool for studying the role of SMAD4 in various biological processes, offering reliable and reproducible results in Western blot and immunofluorescence applications. With its superior performance and versatility, this antibody is a valuable asset for advancing research in cell signaling and therapeutic development.
Product Name:
[KO Validated] Smad4 Rabbit Monoclonal Antibody
SKU:
CAB19116
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 320-552 of human Smad4 (NP_005350.1).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of ChromatinCUT&Tag, 10⁵ cells /1 μg
Synonyms:
JIP; DPC4; MADH4; MYHRS; d4
Positive Sample:
293T, HCT 116, HepG2, C6, NIH/3T3, Mouse lung
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
60kDa
Observed MW:
70kDa
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasing blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
Purification Method:
Affinity purification
Gene ID:
4089
Clone Number:
ARC5009-06
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,0.05% BSA,50% glycerol,pH7.3.
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ K562 cells with 1μg Smad4 Rabbit mAb(CAB19116), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of Smad4 in representative gene loci (JUNB), as shown in figure.
