[KO Validated] Smad4 Rabbit Monoclonal Antibody (CAB19116)
- SKU:
- CAB19116
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Product Name: | [KO Validated] Smad4 Rabbit Monoclonal Antibody |
SKU: | CAB19116 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 320-552 of human Smad4 (NP_005350.1). |
Sequence: | PEYW CSIA YFEM DVQV GETF KVPS SCPI VTVD GYVD PSGG DRFC LGQL SNVH RTEA IERA RLHI GKGV QLEC KGEG DVWV RCLS DHAV FVQS YYLD REAG RAPG DAVH KIYP SAYI KVFD LRQC HRQM QQQA ATAQ AAAA AQAA AVAG NIPG PGSV GGIA PAIS LSAA AGIG VDDL RRLC ILRM SFVK GWGP DYPR QSIK ETPC WIEI HLHR ALQL LDEV LHTM PIAD PQPL D |
Tested Applications: | WB IHC-P IP ChIP ELISA CUT&Tag |
Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of ChromatinCUT&Tag, 10⁵ cells /1 μg |
Synonyms: | JIP; DPC4; MADH4; MYHRS; d4 |
Positive Sample: | 293T, HCT 116, HepG2, C6, NIH/3T3, Mouse lung |
Conjugate: | Unconjugated |
Cellular Localization: | Cytoplasm, Nucleus. |
Calculated MW: | 60kDa |
Observed MW: | 70kDa |
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasing blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
Purification Method: | Affinity purification |
Gene ID: | 4089 |
Clone Number: | ARC5009-06 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,0.05% BSA,50% glycerol,pH7.3. |