The SMAD3 Rabbit Monoclonal Antibody (CAB19115) is specifically designed for research involving SMAD3, a critical protein involved in the TGF-β signaling pathway and essential for various cellular processes such as cell growth, differentiation, and apoptosis. This antibody, raised in rabbits, has been validated for use in techniques such as Western blot and immunohistochemistry, providing reliable detection and analysis of SMAD3 protein expression in human samples.SMAD3 is a key transcription factor that plays a crucial role in mediating TGF-β signaling, which is important for cellular responses such as cell proliferation, differentiation, and migration.
Dysregulation of SMAD3 has been associated with various diseases including cancer, fibrosis, and cardiovascular disorders, making it a promising target for therapeutic interventions. Understanding the function and regulation of SMAD3 is essential for unraveling the complex mechanisms underlying these diseases and developing effective treatment strategies.
Product Name:
[KO Validated] Smad3 Rabbit Monoclonal Antibody
SKU:
CAB19115
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Smad3 (NP_005893.1).
The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3.
Purification Method:
Affinity purification
Gene ID:
4088
Clone Number:
ARC53861
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Smad3 Rabbit mAb (CAB19115) at 1:20000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
