The KO Validated SMAD2 Rabbit Monoclonal Antibody (CAB19114) is a cutting-edge tool designed for research involving SMAD2, a key player in signal transduction pathways regulating cell growth and differentiation. This antibody, manufactured by Assay Genie, is specifically raised in rabbits and has been extensively validated for use in various applications, including Western blotting and immunofluorescence.SMAD2 is a critical mediator in the transforming growth factor-beta (TGF-β) signaling pathway, which plays a crucial role in cell proliferation, differentiation, and apoptosis. Dysregulation of SMAD2 signaling has been implicated in a variety of diseases, including cancer, fibrosis, and developmental disorders.
By using the SMAD2 Rabbit Monoclonal Antibody, researchers can accurately detect and analyze SMAD2 protein levels in different cell types, providing valuable insights into its functional role in physiological and pathological processes.With its high specificity and sensitivity, the SMAD2 Rabbit Monoclonal Antibody is a valuable tool for scientists working in the fields of cell biology, molecular biology, and cancer research. By understanding the intricate mechanisms of SMAD2 signaling, researchers can uncover new therapeutic targets and develop novel strategies for treating diseases associated with aberrant TGF-β signaling. Order your CAB19114 antibody today and take your research to the next level.
Product Name:
[KO Validated] Smad2 Rabbit Monoclonal Antibody
SKU:
CAB19114
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Smad2 (Q15796).
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
4087
Clone Number:
ARC0343
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Smad2 knockdown (KD) HeLa cells, using [KD Validated] Smad2 Rabbit mAb (CAB19114) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
