The SMAD2 Polyclonal Antibody (CAB7699) is a valuable tool for research involving SMAD2, a key protein involved in the TGF-beta signaling pathway. This antibody, produced in rabbits, has been rigorously validated for use in Western blot applications and is highly reactive with human samples.SMAD2 is a critical component in the TGF-beta pathway, playing a crucial role in regulating cell growth, differentiation, and apoptosis. Dysregulation of the TGF-beta pathway, including SMAD2, has been implicated in various diseases, including cancer, fibrosis, and developmental disorders.
By using the SMAD2 Polyclonal Antibody, researchers can explore the role of SMAD2 in these diseases and potentially identify novel therapeutic targets.This antibody enables the detection and analysis of SMAD2 protein levels in various cell types, making it a versatile tool for studies in cell biology, cancer research, and drug development. Understanding the function and regulation of SMAD2 is essential for advancing our knowledge of disease mechanisms and developing targeted therapies for a range of pathological conditions.
Product Name:
[KO Validated] Smad2 Rabbit Polyclonal Antibody
SKU:
CAB7699
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Smad2 (NP_005892.1).
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
4087
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (CAB7699) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5min.
