The SIRT3 Polyclonal Antibody (CAB5718) is a valuable tool for researchers studying SIRT3, a key protein involved in regulating cellular metabolism and aging. This antibody, generated in rabbits, has been validated for use in various applications, including Western blotting. It specifically binds to the SIRT3 protein, allowing for accurate detection and analysis in different cell types.SIRT3, a member of the sirtuin family of proteins, plays a crucial role in maintaining cellular homeostasis by regulating processes such as mitochondrial function, oxidative stress response, and energy metabolism.
Dysregulation of SIRT3 has been linked to various diseases, including cancer, metabolic disorders, and neurodegenerative conditions. By studying the function and expression of SIRT3, researchers can gain insights into the mechanisms underlying these diseases and potentially identify therapeutic targets.Overall, the SIRT3 Polyclonal Antibody (CAB5718) is a reliable tool for investigating the role of SIRT3 in health and disease, offering valuable insights that may lead to the development of novel treatments and interventions in the future.
Product Name:
[KO Validated] SIRT3 Rabbit Polyclonal Antibody
SKU:
CAB5718
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 299-399 of human SIRT3 (NP_036371.1).
SIRT3 encodes a member of the sirtuin family of class III histone deacetylases, homologs to the yeast Sir2 protein. The encoded protein is found exclusively in mitochondria, where it can eliminate reactive oxygen species, inhibit apoptosis, and prevent the formation of cancer cells. SIRT3 has far-reaching effects on nuclear gene expression, cancer, cardiovascular disease, neuroprotection, aging, and metabolic control.
Purification Method:
Affinity purification
Gene ID:
23410
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using [KO Validated] SIRT3 Rabbit pAb (CAB5718) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
