[KO Validated] Sonic Hedgehog (Shh) Rabbit Polyclonal Antibody (CAB18020)
The KO Validated SHH Rabbit Polyclonal Antibody (CAB18020). This antibody is specifically designed for research involving the Sonic Hedgehog (SHH) protein, a key player in embryonic development and cell differentiation processes. Raised in rabbits, this antibody exhibits high reactivity with samples from knockout (KO) experiments, ensuring accurate and reliable results. Validated for use in various applications, including Western blotting, the SHH antibody binds specifically to the SHH protein, enabling precise detection and analysis in different cell types.The SHH protein is known for its pivotal role in regulating cell growth, patterning, and organ development, making it a crucial target for studies in developmental biology, cancer research, and regenerative medicine.
Understanding the mechanisms by which SHH functions can provide insights into various diseases and aid in the development of targeted therapies.Overall, the KO Validated SHH Rabbit Polyclonal Antibody (CAB18020) offers a valuable tool for researchers interested in unraveling the complex functions of the SHH protein and its implications in various biological processes and diseases.
Product Name:
[KO Validated] Sonic Hedgehog (Shh) Rabbit Polyclonal Antibody
SKU:
CAB18020
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 231-330 of human Sonic Hedgehog (Shh) (NP_000184.1).
This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.
Purification Method:
Affinity purification
Gene ID:
6469
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Sonic Hedgehog (Sonic Hedgehog (Shh)) knockout (KO) HeLa cells, using [KO Validated] Sonic Hedgehog (Shh) Rabbit pAb (CAB18020) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
