The SEPHS1 Polyclonal Antibody (CAB19919) is a valuable tool for researchers studying SEPHS1, an essential selenoprotein involved in selenium metabolism and antioxidant defense. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the SEPHS1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for studies in biochemistry, molecular biology, and oxidative stress research.
SEPHS1 plays a crucial role in selenium incorporation into selenoproteins, which are key players in antioxidant defense and redox homeostasis. Understanding the function of SEPHS1 is essential for unraveling the complex mechanisms underlying selenium biology and its impact on various physiological processes. Research into SEPHS1 has implications for studying diseases linked to selenium deficiency, oxidative stress, and metabolic disorders, providing insights for developing novel therapies and interventions.
Product Name:
[KO Validated] SEPHS1 Rabbit Polyclonal Antibody
SKU:
CAB19919
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-392 of human SEPHS1 (NP_036379.2).
This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons.
Purification Method:
Affinity purification
Gene ID:
22929
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and SEPHS1 knockout (KO) 293T cells, using [KO Validated] SEPHS1 Rabbit pAb (CAB19919) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
