Anti-S100A6 Antibody (CAB4104)

Antibody Name:	Anti-S100A6 Antibody [KO Validated]
Antibody SKU:	CAB4104
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human S100A6 (NP_055439.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, NIH/3T3, HT-29, U-251MG, Mouse lung, Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human S100A6 (NP_055439.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MACP LDQA IGLL VAIF HKYS GREG DKHT LSKK ELKE LIQK ELTI GSKL QDAE IARL MEDL DRNK DQEV NFQE YVTF LGAL ALIY NEAL KG
Gene ID:	6277
Uniprot:	P06703
Cellular Location:	Cell membrane, Cytoplasm, Cytoplasmic side, Nucleus envelope, Peripheral membrane protein
Calculated MW:	10kDa
Observed MW:	10kDa

Synonyms:

S100A6, 2A9, 5B10, CABP, CACY, PRA

Background:

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.

UniProt Protein Function:	S100A6: May function as calcium sensor and contribute to cellular calcium signaling (Potential). May function by interacting with other proteins and indirectly play a role in the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative. Belongs to the S-100 family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 1q21 Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; nuclear envelope; nucleus; perinuclear region of cytoplasm; ruffle Molecular Function:calcium ion binding; calcium-dependent protein binding; protein binding; protein homodimerization activity; tropomyosin binding Biological Process: signal transduction
NCBI Summary:	The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma. [provided by RefSeq, Jul 2008]
UniProt Code:	P06703
NCBI GenInfo Identifier:	116509
NCBI Gene ID:	6277
NCBI Accession:	P06703.1
UniProt Secondary Accession:	P06703,Q5RHS4, D3DV39,
UniProt Related Accession:	P06703
Molecular Weight:	10,180 Da
NCBI Full Name:	Protein S100-A6
NCBI Synonym Full Names:	S100 calcium binding protein A6
NCBI Official Symbol:	S100A6
NCBI Official Synonym Symbols:	2A9; PRA; 5B10; CABP; CACY
NCBI Protein Information:	protein S100-A6
UniProt Protein Name:	Protein S100-A6
UniProt Synonym Protein Names:	Calcyclin; Growth factor-inducible protein 2A9; MLN 4; Prolactin receptor-associated protein; PRA; S100 calcium-binding protein A6
Protein Family:	Protein
UniProt Gene Name:	S100A6
UniProt Entry Name:	S10A6_HUMAN

	Western blot analysis of extracts of various cell lines, using S100A6 antibody (CAB4104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts from normal (control) and S100A6 knockout (KO) HeLa cells, using S100A6 antibody (CAB4104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat cerebellum using [KO Validated] S100A6 Rabbit pAb (CAB4104) at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 200ug extracts of U-251MG cells using 3ug S100A6 antibody (CAB4104). Western blot was performed from the immunoprecipitate using S100A6 antibody (CAB4104) at a dilition of 1:1000.

Antibodies	ELISA Kits
S100A6 Antibody (PACO12007)	Human S100A6 ELISA Kit
	Human S100A6 (S100 Calcium Binding Protein A6) CLIA Kit (HUES00747)
	Human S100A6 (S100 Calcium Binding Protein A6) ELISA Kit (HUES02351)
	Human Protein S100-A6 (S100A6) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)