The RPL22 Polyclonal Antibody (CAB19866) is a powerful tool for researchers studying ribosomal protein L22 (RPL22) and its role in various cellular processes. This antibody, produced in rabbits, has been validated for use in techniques such as Western blotting, immunohistochemistry, and immunofluorescence, allowing for accurate detection and analysis of RPL22 in different experimental settings.RPL22 is a key component of the ribosome, playing a crucial role in protein synthesis and cell growth. Dysregulation of RPL22 has been linked to various diseases, including cancer, making it an attractive target for investigation in oncology research.
By using the RPL22 Polyclonal Antibody, researchers can gain insight into the expression levels and localization of RPL22 in cells and tissues, providing valuable information for understanding its function and potential therapeutic implications.Overall, the RPL22 Polyclonal Antibody is a valuable resource for studying the biology of RPL22 and its involvement in disease processes, offering researchers a reliable tool for advancing their research in molecular biology and medicine.
Product Name:
[KO Validated] RPL22 Rabbit Polyclonal Antibody
SKU:
CAB19866
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human RPL22 (NP_000974.1).
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2.
Purification Method:
Affinity purification
Gene ID:
6146
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and RPL22 knockout (KO) 293T cells, using [KO Validated] RPL22 Rabbit pAb (CAB19866) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90S.
