The ROCK1 Polyclonal Antibody (CAB1008) is a powerful tool for researchers studying the Rho-associated protein kinase 1 (ROCK1) signaling pathway. This antibody, produced in rabbits, is highly specific to human ROCK1 protein and has been validated for use in Western blot applications. By targeting ROCK1, researchers can explore its role in various cellular processes, including cell migration, cytoskeletal dynamics, and cell proliferation.ROCK1 is a key regulator of actin cytoskeleton dynamics and is involved in numerous cellular functions, such as cell adhesion and cell motility.
Dysregulation of the ROCK1 pathway has been implicated in various diseases, including cancer, cardiovascular diseases, and neurological disorders. Therefore, studying the activity of ROCK1 using this antibody can provide valuable insights into the pathogenesis of these diseases and potentially identify novel therapeutic targets.Overall, the ROCK1 Polyclonal Antibody (CAB1008) is an essential tool for researchers interested in unraveling the complex mechanisms of ROCK1 signaling and its impact on cellular processes and disease development.
Product Name:
[KO Validated] ROCK1 Rabbit Polyclonal Antibody
SKU:
CAB1008
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
Purification Method:
Affinity purification
Gene ID:
6093
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KO Validated] ROCK1 Rabbit pAb (CAB1008) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
